Surface of explants of transgenic plants are sterilized using dilute sodium hypochlorite and are aseptically transferred to culture vessels containing nutrients and plant growth regulators.
Cytokinin is added to nutrient agar to stimulate the cells of explant to divide by mitosis forming a callus and these cells are induced to proliferate into particular tissues by varying the combination of the various plant growth regulators.
The number of calli increased by subculturing and the cells of the callus are induced by auxin and giberellin to differentiate into particular tissues.
When these tissues developed into a plantlet and become suffieciently large, it is transferred from a laboratory to the soil and grown into a whole plant.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment